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The research on comparing the water quality in natural ponds and concrete ponds at Wichienmatu School, Trang Province, aims to study the water quality in these two types of ponds within the school. The water quality was assessed by measuring pH levels, water temperature, water transparency, and dissolved oxygen content. The experiment found that The study found that the water quality in the natural pond and the concrete pond differs significantly. The pH level in the natural pond was 7.6, indicating a slightly alkaline condition, while the pH level in the concrete pond was 7.0, which is neutral. However, both values remain within a suitable range for aquatic life. Regarding water temperature, the concrete pond had a higher average temperature of 32.33°C, compared to 28.33°C in the natural pond. This difference may be due to the concrete pond’s ability to absorb and retain heat more effectively than the natural pond. Additionally, the dissolved oxygen (DO) concentration was higher in the concrete pond, averaging 3.67 mg/L, while the natural pond had a lower average of 2.0 mg/L. This difference could result from variations in water circulation and biological composition between the two ponds. Meanwhile, water transparency was greater in the natural pond, suggesting a lower presence of suspended solids and microorganisms compared to the concrete pond. Based on the findings, it can be concluded that the natural pond provides a more balanced biological environment as it supports the survival of aquatic organisms under natural conditions. However, the natural pond tends to contain more suspended solids and organic matter, which may impact water quality and cleanliness. If the water is to be used for consumption, additional treatment may be required to ensure its suitability for use.