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This study aimed to compare water quality and plant diversity in natural canals at Yan Ta Khao and irrigation canals at Na Po, Trang Province, Thailand. The instruments used included water transparency measurement using a Transparency Tube in centimeters ( cm) , comparison of dissolved oxygen levels using a Dissolved Oxygen (DO) kit, and comparison of water temperatures using a thermometer and a pH paper device to measure pH. The results of the study found that: The results of the study of water from both sources at the natural canal in Yan Ta Khao Sub-district, Yan Ta Khao District, Trang Province, Thailand and the irrigation canal at Ban Na Po, Khuan Pring Sub-district, Mueang District, Trang Province, Thailand were slightly different, namely, the transparency of water at the natural canal and the irrigation canal had an average of 73 cm and the measurement of oxygen content in the water from both sources had an average of 7.2 mg/L and the temperature measurement in the water from both sources were different, the natural canal had an average of 28.3 °C and the irrigation canal 28.7 °C and the acid-base measurement of the water from both sources had different averages, the natural canal had an average of 6 and the irrigation canal had an average of 7 and the aquatic plants in the natural canal were as follows: stevia and the irrigation canal were as follows: algae