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Currently, Azolla (Azolla microphylla) is popular in the field of trade, and it can be used as animal feed or consumed in households because of its low cultivation cost. However, the cultivation method is not difficult and can propagate quickly and yields high yields, but the cultivation of Azolla has some limitations, such as the uncertain increase in the amount of Azolla and pests. Acidity-base (pH), dissolved oxygen content (DO), amount of light, etc. Therefore, studying the factors that affect the increase in the number of Azolla is important for farmers who cultivate Azolla. Recognizing this importance, the organizers studied the relationship between environmental factors and the density of the Azolla population in 8 ponds. The average pH was 6.09, the average temperature was 27.65 °C, the average TDS was 54.83 ppm, the EC was 87.83 μs/cm, the salinity was 48 ppm, the DO was 3.9 mg/L, and the water translucency was 23.14 cm. The average pH was 5.71, the average temperature was 27.67 °C, the average TDS was 57.49 ppm, the EC was 149.8 μs/cm, the salinity was 57 ppm , the DO was 5.1 mg/L, and the water translucency was 35.18 cm. It was found that the average EC, average DO, average Salt and average water translucency in ponds with dense Azolla populations were lower than in ponds with light Azolla populations, while the average pH, average temperature and TDS values in ponds with dense Azolla populations and ponds with light Azolla populations were similar. Based on the estimation of the population density of Azolla by the plot-based sampling method. The Quadrat Sampling Method showed that ponds 1-4 were ponds with dense Azolla populations with estimated population densities of 7,300, 6,900, 7,800 and 9,800 plants/square meter, respectively, and ponds 5-8 were ponds with lightAzolla populations with 2,575, 1,206, 331 and 672 plants/square meter, respectively. The results of the study showed that the physical properties were EC , DO, Salt and water translucency. It affects the density of the Azolla population.