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The purpose of this study was to make a water quality comparison between the upstream point of Huai Sai Waterfall and the downstream point of Nong Nam Khiao Reservoir, Chonburi. The operation was divided into 3 steps. The first step was to determine the water sample points from Nong Nam Khiao Reservoir and Huay Had Sai Waterfall, 4 sample points in each place. The second step was collecting water samples and testing the water quality. This step has two parts: First, Testing the quality at the actual area by using a Multiparameter water quality instrument. Next part, sent to the laboratory for testing. Last step was field or environmental data collection. From the study, We found that At Huai Had Sai Waterfall, the temperature was 26.3 - 26.7 °C, pH was 6.09 to 6.78, dissolved oxygen was 3.33 – 4.8 mg/L, Conductivity and dissolved solid were 189.8 – 274.9. µS/cm, ammonium content The nitrate and phosphate values were between <0.2 – 0.2 mg/L, 0 mg/L and 0.025 – 0.6 mg/L, respectively. The temperature of Nong Khiaw Reservoir was 32.8 to 34.7 °C, pH was 6.99 to 7.98, the dissolved oxygen content was 3.32 – 5.70 mg/L, conductivity and dissolved solids were 105.30 – 155.35 µS/cm, ammonium content. The nitrate and phosphate values were between 0.4 - 0.6 mg/L, 0 mg/L and 0.025 - 0.04 mg/L, respectively. The experiments showed that the water quality at Huay had Sai Waterfall was better than the Nong Nam Khaio reservoir because the ammonium content, Conductivity and dissolved solids were significantly less.