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Plants often lack essential nutrients, necessitating the development of fertilizers that provide adequate nourishment while allowing for nutrient adjustments suitable for both soil and crops. This study investigated the optimal ratio of shrimp shell waste to tamarind seed gelose at 1:1, 2:1, and 3:1, confirming that all ratios could form solid granules. The researchers selected the 3:1 ratio for experimentation. Using a Scanning Electron Microscope (SEM) to analyze the fertilizer's composition, key plant macronutrients were identified: nitrogen (N) at 9.70±2.41%, phosphorus (P) at 2.44±0.29%, and potassium (K) at 0.92±0.08%. The micronutrients detected included magnesium (Mg) at 0.60±0.07%, calcium (Ca) at 8.86±1.69%, and silicon (Si) at 0.48±0.13%. In a controlled study, RD79 rice (Oryza sativa L.) was grown with varying fertilizer application rates. The rice plants that received 8 grams of the biogranular shrimp shell fertilizer exhibited the best growth at 50 days, reaching an average height of 70.36±0.80 cm, with a dry weight of 2.31±0.06 g and an average of 11.36±1.12 leaves per plant. Soil fertility was assessed using an N-scale with three levels: Too Little (N), Ideal (P), and Too Much (M). The soil treated with 8 grams of the shrimp shell fertilizer coated with tamarind seed gelose showed the most favorable chemical properties for plant growth. When RD79 rice was cultivated in real farming conditions, plant growth varied. By the third growth stage, rice plants treated with the bio-fertilizer reached a greater height of 131.70±0.96 cm, with a dry weight of 8.09±0.07 g and an average of 25.00±1.24 leaves per plant. Additionally, yield analysis revealed differences among treatments. The RD79 rice treated with the bio-fertilizer at the third growth stage produced grains with an average 100-grain weight of 3.11±0.13 g, an average of 131.00±2.00 grains per panicle (based on 10 panicles), and an average 100-grain length of 1.06±0.05 cm.